A rapid and precise method for the determination of urea.
نویسندگان
چکیده
A method is described for the determination of urea in plasma and urine. The effects of variations in the experimental conditions are examined and the results of recovery experiments and other tests of precision and accuracy are reported. In comparison with other methods in current use, this method has distinct advantages in sensitivity, simplicity, and precision, thus economizing in time, sample volume, reagents, and equipment.
منابع مشابه
KINETIC-SPECTROPHOTOMETRIC DETERMINATION OF TRACE AMOUNTS OF NITRITE BY THE REACTION OF METHYL ORANGE FORMATION
The composite diazotization coupling reaction of sulfanilic acid and N, Ndimethylaniline was used for the precise, accurate, and rapid determination of trace amounts of nitrite ion (0.02-3 µg/ml). The reaction was monitored spectrophotometrically by measuring the absorbance of the produced methyl orange at 510 nm by a fixed the method of 5.0 minutes. The relative standard deviation for the...
متن کاملSimple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کاملSimple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کاملAn Improved GC Method for Rapid Analysis of Valproic Acid in Human Plasma Without Derivatization
A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®<span styl...
متن کاملSelective and Validated SpectrophotometricMethods for Determination of Acyclovir and Ganciclovirwith 2, 4 as Reagent
New, simple and sensitive spectrophotometric methods for the determination of acyclovir and ganciclovirhave been developed. The method is based on the oxidation of 2, 4-dinitrophenyl hydrazine (2, 4 DNP) and coupling of the oxidized product with acyclovir and ganciclovirto give intensely coloredchromogen. Acyclovir and ganciclovirshowed maximum absorbance at 414 nm and 450 nm with linearity was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 13 شماره
صفحات -
تاریخ انتشار 1960